FIG. 5.

Expression of the secretory form of gD. The culture fluids from COS7 cells transfected with pBgD (lanes 1 and 4), pBgDdTM (lanes 2 and 5), or pcDNA3.1(−) (lanes 3 and 6) and then labeled with [³⁵S] were subjected to SDS-PAGE directly (lanes 4 to 6) or after immunoprecipitation with anti-gD(SA8) serum (lanes 1 to 3). The position of gDdTM is indicated on the left, and molecular mass standards (in kilodaltons) are shown on the right.