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Detection of antibody to the secretory form of gD, gDdTM, in monkey sera by the dot blot method. The position of the purified gDdTM (a), culture supernatant from COS7 cells transfected with pBgDdTM (b), or vector DNA (c) on each membrane is schematically indicated in the inset box. After blocking, each membrane was incubated with individual monkey serum.
