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. 2022 Jan 27;9:758968. doi: 10.3389/fmolb.2022.758968

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Copyright © 2022 Wang, Zhao, Zhang, Jiang, Ma, Zhang and Dong.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Protein expression and function investigation of USF1 in LUAD. (A) The USF1 protein expression in the studied tissues were determined by immunohistochemical assay; (B) The protein expression of USF1 in cells were determined by WB; (C,D) Transfection efficiency was proved by WB in H1299 and A549 cells; (E,F) Cells viability was detected by CCK-8 assay in H1299 and A549 cells; (G,H) cells proliferation potential was detected by the colony forming assay in H1299 and A549 cells. (NC group, cells were transfected with si-NC; si-USF1 group cells were transfected with si-USF1; *p < .05 means statistically significant difference when compared with NC group).