TABLE 2.
Oligonucleotide primers used for PCR to amplify partial citrate synthase gene
| Primer | Sequence (5′-3′)a | Organisms in which primer is used |
|---|---|---|
| Forward | ||
| F3 | TCT-TCT-CAT-CCT-ATG-GC | HGE agent |
| F4b | CCG-GGT-TTT-ATG-TCT-ACT-GC | HGE agent, E. equi, E. phagocytophila, A. marginale South Idaho, A. marginale Florida, E. chaffeensis, E. muris, C. ruminantium |
| F4e | ACT-GCT-TCK-TGT-SAR-TC | N. helminthoeca |
| F1 | CAT-GAR-CAR-AAT-GCT-TC | E. sennetsu |
| Chaf-F | GGA-TTA-TGG-TWR-AAR-AAG-C | E. canis |
| EH130F | GGW-TTY-ATG-TCY-ACT-GCT-GC | A. centrale |
| SEN330F | AAA-TAT-CCG-TTC-TTT-CCC-AAC-G | E. risticii |
| Reverse | ||
| R1b | CGA-TGA-CCA-AAA-CCC-AT | HGE agent, E. equi, E. phagocytophila, A. marginale South Idaho, A. marginale Florida, E. chaffeensis, E. muris, C. ruminantium, N. helminthoeca, E. sennetsu |
| Chaf-R | TAY-AAC-TGR-CGT-GGR-CG | E. canis |
| HG1085R | ACT-ATA-CCK-GAG-TAA-AAG-TC | A. centrale |
| SEN890R | GCT-TTA-ATA-TGG-CTG-CAC-GAG | E. risticii |
a
S, G or C; R, A or G; W, A or T; Y, C or T; K, G or T.