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. 2022 Jan 27;12:818468. doi: 10.3389/fmicb.2021.818468

TABLE 2.

A summary of the details of a collection of published bile tolerance assays.

Genus Material Concentration (%) Type of bile Duration (h) Detection References
L. rhamnosus Freeze-dried 1 Porcine bile 1 Plate count enumeration Stage et al., 2020
L. casei Fresh cells 0.1 Cholate/deoxycholate 1 Plate count enumeration Huang et al., 2018
Different LAB Fresh cells 0.3 Ox bile salts 1, 2, 3, 4, 5, and 6 OD600 Ilango et al., 2016
Different LAB Spray-dried 3.6 Ox bile salts 2 and 4 Plate count enumeration Ilango et al., 2016
Different LAB Freeze-dried 3.6 Ox bile salts 2 and 4 Plate count enumeration Ilango et al., 2016
B. animalis Fresh cells 0.3, 0.5, and 1 Ox bile salts 4 Plate count enumeration Charnchai et al., 2016
L. plantarum Spray-dried 2 1, 2, 3, and 4 Plate count enumeration Dolly et al., 2011
L. plantarum Freeze-dried 2 1, 2, 3, and 4 Plate count enumeration Dolly et al., 2011
Different LAB Fresh cells 0.3 Oxgall 0.5, 1, 1.5, 2, 3, 4, 5, 6, 7, and 24 OD620 Jacobsen et al., 1999
Different LAB Fresh cells 0.3 Oxgall 4 Plate count enumeration Jacobsen et al., 1999
Different LAB Fresh cells 0.3, 0.6, 0.8, 1, 2, and 5 Oxbile 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11 OD600 Brink et al., 2006
L. casei Spray-dried 1, 2, and 3 Oxgall 0.5, 1, 2, and 3 Plate count enumeration Gul and Atalar, 2019
L. casei Freeze-dried 1, 2, and 3 Oxgall 0.5, 1, 2, and 3 Plate count enumeration Gul and Atalar, 2019
L. plantarum Freeze-dried 3 Taurocholic acid 4 and 8 Plate count enumeration Tee et al., 2013
L. reuteri Fresh cells 0.3 Oxgall 16 Continuous OD measurements Taranto et al., 2003
L. reuteri Freeze-dried 0.5 Porcine bile 30 min intervals FC Hernández et al., 2019
L. reuteri Freeze-dried 1 Bovine bile 30 min intervals FC Hernández et al., 2019
L. reuteri Fresh cells 6 Bovine bile 72 Plate count enumeration Rosander et al., 2008