Fig. 1.

Hyperglycemia increases neuronal ferroptosis and aggravates acute outcomes after ICH. (A–F) Brain slices obtained at day 1 and 3 post ICH were stained with FJC (A, B), MDA and NeuN (C, D), or CV/Luxol fast blue (E, F). (A, B) FJC staining represents degenerating neurons. The high power image of FJC⁺ cell was shown in the white box. White arrows indicate FJC⁺ cells. (C, D) Immunofluorescence of MDA and NeuN was performed. Representative images were shown at 1-day post-ICH. The high-power image of a NeuN⁺MDA⁺ cell was shown in the white box. White arrows indicate double positive cells. The mean fluorescence intensity of MDA was quantified (D). (E, F) Lesion volume (black dotted bordered) was calculated by CV/Luxol fast blue staining. (G–J) Acute outcomes were examined up to 7 days after ICH. (B, D, F-J) Two-way ANOVA followed Tukey's multiple comparisons test. *p < 0.05, **p < 0.01, ***p < 0.001 vs corresponding Sham; #p < 0.05, ##p < 0.01 vs Veh + ICH. Each group contained 6–7 (B), 6 (F), 4 (D), and 10–30 (G–J) animals. Scale bar: (A) 50 μm, (C) 75 μm, (E) 1 mm. Each experiment was repeated at least 3 times independently. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)