Fig. 6.

Ltf rescues neuronal ferroptosis and improved acute outcomes in hyperglycemic ICH mice. (A–G) Ltf-siRNA or Scr-siRNA was injected intraventricularly to normoglycemic ICH mice. (A, B) Immunofluorescent staining using antibodies of MDA and NeuN. White arrows indicate the MDA⁺NeuN⁺ cells (A). The MFI of MDA was quantified (B). (C, D) FJC staining was performed and the FJC⁺ cells were quantified. White arrows indicate FJC⁺ cells. (E, F) CV/Luxol fast blue staining were performed to quantify the lesion volume (black dotted bordered). (G) Neurological function and motor function were examined following ICH blindly. (H–N) Four mg/kg of rLtf was administered (i.p.) to hyperglycemic ICH mice. Immunofluorescent staining using antibodies of MDA and NeuN (H, I), FJC staining (J, K), and CV/Luxol fast blue staining (L, M) were performed. The representative images (H, J, L) and quantifications (I, K, M) are shown. Neurological function and motor function were examined following ICH blindly (N). (B, D, F, I, K, M) Student's t-test followed by Welch’s correction. (G, N) Two-way ANOVA followed by Tukey's multiple comparisons test. *p < 0.05, **p < 0.01, ***p < 0.001 vs corresponding Scr-siRNA (B, D, F, G) or corresponding Veh (I, K, M, N). Each group contained 6 (B, D, F, I, K, M) or 8 (G, N) animals. Scale bar: (A, H) 75 μm, (C, J) 50 μm, (E, L) 1 mm. Each experiment was repeated at least 3 times independently. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)