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. 2022 Jan 27;12:807514. doi: 10.3389/fpls.2021.807514

FIGURE 5.

FIGURE 5

Overexpression of CsPMEI4 promotes early flowering in transgenic Arabidopsis under LD condition. (A) The expression of CsPMEI4 in three CsPMEI4-OE lines and WT plants. The relative expression levels were calculated by using 2– Δ Ct method. Data are shown as the means ± SE (n = 3). (B) Early flowering phenotypes of CsPMEI4-OE lines grown under LD condition. (a) Early-flowering phenotype; (b) phenotypes of CsPMEI4-OE lines and WT plants after 30 d of growth under LD condition; (c) leaf size; (d) phenotype of CsPMEI4-OE lines and WT plants after 40 days of growth under LD condition; (e) phenotype of CsPMEI4-OE lines and WT plants at senescence stage; (f) flowers sizes of WT and CsPMEI4-OE lines; (g) seed size of WT and CsPMEI4-OE lines. (C) Flowering time, rosette leaves numbers, and plant height of WT and CsPMEI4-OE lines at the just bolting stage. (a) Flowering time; (b) rosette leaves numbers; (c) plant height. (D) Expression analysis of flowering-related genes. The final results were calculated with 2– Δ Δ Ct method. Data are shown as the means ± SE (n = 3).