Figure 11.

¹H NMR spectra of Aβ(1–16) showing valine methyl group resonance shifts during (A) Zn²⁺ and (B) Cd²⁺ titration. Increasing molar equivalents of metal ion titrated resulted in larger chemical shift changes, indicating stronger binding. In (C), the plot of valine γCH3 chemical shifts (ppm) against the molar equivalents of Zn²⁺ and Cd²⁺ relative to Aβ shows increasing interactions of the peptide with metal up to equimolar ratios, indicating a 1:1 stoichiometry. Insert indicates chemical shifts for the γ and γ’ methyl protons of valine. Adapted with permission from Syme and Viles [223], Copyright (2006) Elsevier.