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. 2022 Jan 27;13:773001. doi: 10.3389/fimmu.2022.773001

Figure 4.

Figure 4

CBP is recruited by STAT1-IRF1 to upregulate XOR expression in HepG2 cells. (A) Analysis of the XDH promoter sequence using transcription factor affinity prediction (TRAP) web tools (http://trap.molgen.mpg.de/cgi-bin/trap_multi_seq_form.cgi). (B, C) XOR mRNA and protein expression, after treatment with siNC or siIRF1 for 48 h; (D) IRF1, (E) CBP and H3K27ac, (G) STAT1 enrichment after treatment with siNC, siIRF1, siSTAT1 for 48 h or with siCBP for 24 h; (F) CBP, XOR, STAT1 and IRF1 protein expression after treatment with siNC or siCBP for 24 h; (H) CBP, p-STAT1, STAT1, and IRF1 protein expression after treatment with siNC, siSTAT1 or siIRF1 for 48 h, in HepG2 cells treated with or without IFN-γ (10 nM). Significance for all data was determined by the independent samples t-test. Data are shown as mean ± S.D., *p < 0.05, **p < 0.01 compared with Control; # p < 0.05, ## p < 0.01, ### p < 0.001 compared with IFN-γ.