Figure 7.

PIC rescued HG/HL-induced cytoskeletal reorganisation and dysfunction of cytoskeleton-associated protein GTPases (Rac1, Cdc42) and IQGAP1. A. Immunofluorescence staining of F-actin showing that HG/HL treatment resulted in a thickened F-actin band and the presence of F-actin bundles inside the cells. PIC ameliorated the actin cytoskeleton remodelling and maintained cell morphology. B. Western blotting analysis showing the expression of cytoskeleton-associated proteins, Rac1/Cdc42, and their effector, IQGAP1. C-E. The levels of these proteins relative to GAPDH. Values are expressed as means ± SD. ***P < 0.05, vs. control group; ###P < 0.05, vs. HG/HL treatment group.