Effects of PIC on HG/HL-stimulated NF-κB translocation and ROS production. (A) Intracellular ROS production in HUVECs based on the fluorescent probe, DCFH-DA, and (B) fluorescence intensity analysed using ImageJ. (C) NF-κB nuclear translocation during its activation evaluated using immunofluorescence staining, and (D) fluorescence intensity analysed using ImageJ. Values are expressed as means ± SD. ***P < 0.05, vs. control group; ###P < 0.05, vs. HG/HL treatment group. Scale bar: 200 μm for 4×, 100 μm for 10× and 50 μm for 20×.