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. 2021 Dec 20;57:101421. doi: 10.1016/j.molmet.2021.101421

Figure 2.

Figure 2

Mitochondria in close proximity to clusters of insulin vesicles in β-cells stimulated with glucose. Orthoslices from two representative cells (two separate cells shown in A and B, respectively) stimulated with 25 mM glucose for 30 min are displayed. To assist in tracking the cellular orientation between the XY, YZ, and XZ views, a single cluster of secretory vesicles is highlighted in red in each orthoslice. Nearby mitochondria are indicated by black arrows. The position of orthoslices for XY, YZ, and XZ views are indicated in the far-right panel showing the 3D segmentations. The red box indicates the XY view, the blue box indicates the YZ view, and the green box indicates the XZ view. Images with scale bars were generated in Fiji (Image J) and XY, YZ, XZ slices, and 3D orientations were generated in Amira Software (FEI). Modified from [16].