Figure 4.

Osteoblastic cells express EMT- and MET-associated genes

(A) Osteoblastic cells colored by epithelial differentiation score (left), mesenchymal differentiation score (second from left), and merged epithelial and mesenchymal differentiation scores (third from left). Color legend at right.

(B) Partial EMT and MET result in intermediate cellular phenotypes that encompass dual epithelial and mesenchymal phenotypes (bottom).

(C) Dotplot of the expression of canonical EMT and MET markers in the osteoblastic subclusters. Clusters were also scored for the expression of EMT and MET gene signatures.

(D–G) Feature plots for esrp1, twist2, cdh11, and zeb2a. (H) Top differentially expressed MET and EMT genes in the osteoblasts as visualized by heatmap. (I) Pseudotemporal expression of MET (top) and EMT (bottom) gene signatures in the osteoblast 2 cells using trajectory 2