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. 2021 Jul 12;93(29):9974–9979. doi: 10.1021/acs.analchem.1c00877

Figure 2.

Figure 2

Bulk quantitation of microfluidic ddPCR products: (a) total fluorescence of ddPCR emulsions measured with a plate reader (Tecan), (b) detection of stained total DNA recovered from ddPCR emulsions (Qubit), (c) quantitation of amplicon peak with gel electrophoresis (Bioanalyzer) of ddPCR emulsions, and (d) qPCR quantitation of ddPCR amplicons. ddPCR+qPCR shifts the qPCR Ct to lower cycles, allowing enhanced sensitivity compared to qPCR alone. n = 3, error bars represent standard deviation.