FIG. 3.

Gel electrophoresis of omp2 PCR products of Chlamydiaceae after restriction digestion with AluI. Lanes 1 and 16, size markers (as in Fig. 2); lane 2, C. trachomatis serotype D; lane 3, C. trachomatis serotype L2; lane 4, C. suis S45; lane 5, C. muridarum MoPn; lane 6, C. pneumoniae TW183; lane 7, C. pneumoniae V1355; lane 8, unidentified isolate A; lane 9, C. psittaci Z10; lane 10, C. abortus AB7; lane 11, C. caviae GPIC; lane 12, C. felis FePn; lane 13, unidentified isolate B; lane 14, C. pecorum P787 (C. pecorum 11/88 and R69 also gave this pattern); lane 15, C. pecorum VR628 (uninfected McCoy cells also gave this pattern). All amplifications were performed with primers Ch1 and Ch2 with an annealing temperature of 55°C except for the C. pecorum strains and uninfected McCoy cells, for which the annealing temperature was 45°C. The predicted sizes (in base pairs) of the digest fragments are listed in Table 2.