TABLE 1.
omp2 primer and probe sequencesa
| Designation | Specificity | Sequence (5′ → 3′) |
|---|---|---|
| Ch1 | Family-specific PCR primer (sense) | ATG TCC AAA CTC ATC AGA CGA G |
| Ch2 | Family-specific PCR primer (antisense) | Bio-CCT TCT TTA AGA GGT TTT ACC CA |
| D2 | Family-specific PCR primer (antisense) | CCT TCT TTA AGA GGT TTT ACC |
| CfamP | Family-specific probeb | GTA GGA TCT CCT TAT CCT ATT G |
| CtraP | C. trachomatis probeb; also detects animal isolates C. muridarum and C. suis | TGA CAC CAA AGC GAA AGA CAA |
| CpnP | C. pneumoniae probeb | AAG GTT AGA CTT GTC CGT AGA |
| Tail sequenceb | GCACAACTCATTATGAA | |
| AP-COM | Common alkaline phosphatase probe | AP-TTCATAATGAGTTGTGC |
a
All oligonucleotides were purchased from Oswel DNA Service, Southampton, England.
b
Each probe was extended at the 3′ end with the same tail sequence.