TABLE 4.

PCR-ELONA limiting dilution titration for C. pneumoniae EB suspension^a

EB input into PCR	ELONA cps				No. positive/4 tested
	Replicate 1	Replicate 2	Replicate 3	Replicate 4
30	197,435	91,228	91,351	169,439	4
10	65,743	15,039	101,287	87,291	4
3	8,027	28,837	34,542	14,435	4
1	549	553	620	14,430	1
0.3	577	616	567	553	0
0.1	609	607	616	666	0

^aPCR was performed with primers Ch1 and Ch2 and an annealing temperature of 55°C.