Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Feb 10;41:59. doi: 10.1186/s13046-022-02258-9

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

PMC Copyright notice

Fig. 2 — HPV16 regulation of SMAD4 expression. A, B HPV-positive HNC cell lines were transfected for 72 h with specific siRNA against HPV16 E6/E7 or Luciferase as control. After transfection cells were lysed and analysed by immunoblotting with the indicated antibodies (A) or total RNAs were isolated for RT-qPCR and reported as means ±SD of fold changes of at least three independent experiments (B). C, D HKs were transduced with empty or HPV16 E6/E7 or HPV11 E6/E7 recombinant retroviral vectors. After selection with G418 cells were harvested. C Lysates were collected and analysed by immunoblotting with the indicated antibodies. D Total RNAs were isolated for RT-qPCR. SMAD4 expression was normalized to RpP0. Results from at least three independent experiments are expressed as means ±SD. E HPV-positive cell lines were transfected with specific siRNA against ΔNp63α or Luciferase as control. 72 h after transfection cells were lysed and analysed by immunoblotting with the indicated antibodies. F, G HPV-positive HNC cell lines were transfected with either specific siRNA against p53 (F) or pCMV expressing p53 (G). Cells were the lysed and analysed by immunoblotting with the indicated antibodies