Figure 9.

Quantifying number of molecules normalized to the membrane surface area and Gaussian curvature analysis along the nanopillars. (A) Distribution of molecules of AP-2 in cells along the nanopillars normalized to the membrane surface area (n = 49). We observe very few relative molecules near the bottom of the coverslip. The relative number is around two from Z = 400 to Z = 800 nm. Near the top of the pillars, the relative number increases substantially. (B) Distribution of actin molecules in cells near the nanopillars normalized to the membrane surface area (n = 27). The number of molecules relative to the membrane is nearly constant until around Z = 800 nm. The relative number increases greatly after this point. (C) Representative mesh surface derived from 3D localization data from the transmembrane-labeled 3D SR reconstruction. (D) Gaussian curvature along the nanopillar. Curvature is nearly 0 but eventually increases to relatively large positive values closer to the cap. Values are mean ± SEM.