| Antibodies |
|
|
|
| HA |
Abcam |
Cat# ab9110; RRID:AB_307019 |
| GFP |
Abcam |
Cat# ab290; RRID:AB_303395 |
| VPS35 |
Abcam |
Cat# ab57632; RRID:AB_946126 |
| VPS26a |
Abcam |
Cat# ab23892; RRID:AB_2215043 |
| VPS26b |
Novus |
Cat# NBP1-92575; RRID:AB_11020053 |
| VPS29 |
Novus |
Cat# NBP1-85288; RRID:AB_11009921 |
| VPS29 |
Sigma-Aldrich |
Cat# sab2501105; RRID:AB_10602838 |
| β-actin |
Abcam |
Cat# ab6276; RRID:AB_2223210 |
| beta III tubulin |
Abcam |
Cat# ab18207; RRID:AB_444319 |
| NeuN |
Abcam |
Cat# ab177487; RRID:AB_2532109 |
| GluA1 |
Millipore |
Cat# mab2263; RRID:AB_1711819 |
| APP & APP-CTF |
Abcam |
Cat# ab32136; RRID:AB_2289606 |
| GFAP |
Abcam |
Cat# ab68428; RRID:AB_1209224 |
| IBA1 |
Abcam |
Cat# ab178846; RRID:AB_2636859 |
| CD11b |
Abcam |
Cat# ab133357; RRID:AB_2650514 |
| Cathepsin D |
Abcam |
Cat# ab19555; RRID:AB_444974 |
| SORL1 |
BD bioscience |
Cat# BD-611861; RRID:AB_399341 |
| GFP (IHC) |
Abcam |
Cat# ab13970; RRID:AB_300798 |
| GluA1 (IHC) |
Abcam |
Cat# ab183797; RRID:AB_2728702 |
| GFAP (IHC) |
Abcam |
Cat# ab7260; RRID:AB_305808 |
|
| Chemicals, peptides, and recombinant proteins |
|
|
|
| Recombinant Protein G Agarose beads |
ThermoFisher |
15920010 |
| LDS Sample Buffer |
ThermoFisher |
NP0007 |
| 10X Reducing Agent |
ThermoFisher |
NP0009 |
| Hoechst 33258 nuclear stain |
ThermoFisher |
H3569 |
| ProLong Gold Antifade Mountant |
ThermoFisher |
P36934 |
|
| Critical commercial assays |
|
|
|
| Pierce BCA protein assay kit |
ThermoFisher |
23225 |
|
| Experimental models: Organisms/strains |
|
|
|
| VPS35 nsKO mice |
This paper and Simoes et al. 2020
|
N/A |
| VPS35 nsKO + Htau mice |
This paper |
N/A |
| VPS35 nsKO + Tau KO mice |
This paper |
N/A |
| C57BL/6J mice |
Jackson Labs |
000664 |
|
| Plasmids and Viral Vectors |
|
|
|
| pcDNA3.1 VPS35-HA |
This paper |
N/A |
| pcDNA3.1 VPS35-GFP |
This paper |
N/A |
| AAV9 VPS35-HA |
This paper |
N/A |
| AAV9 VPS35-GFP |
This paper |
N/A |
| AAV9 GFP |
This paper |
N/A |
| AAV9 Empty Vector |
This paper |
N/A |
|
| Other |
|
|
|
| optimal cutting temperature (OCT) |
Tissue-tek |
62550-12 |
| Superfrost™ Plus Microscope Slides |
Fisher |
12-550-15 |
| Surgipath X-tra Adhesive Microscope slides |
Leica |
3800200 |
