KEY RESOURCES TABLE

REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Anti-p53 (Ab-6) (Pantropic) Mouse mAb (DO1)	Millipore	Cat#OP43; RRID: AB_213402
p21 antibody [EPR362]	Abcam	Cat#ab109520; RRID: AB_10860537
GAPDH antibody	Fitzgerald Industries International	Cat#10R-G109a; RRID: AB_1285808
Mouse Anti-SC35 Monoclonal antibody (against SRRM2)	Abcam; also see Ilık et al., 2020	Cat#ab11826; RRID: AB_298608
p53 antibody	Novus	Cat#NB200-171; RRID: AB_10003046
SON antibody	Abcam	Cat#ab121759; RRID: AB_11132447
Bacterial and Virus Strains
Stbl3 Chemically Competent E. coli	Invitrogen	Cat#C7373-03
Chemicals, Peptides, and Recombinant Proteins
Nutlin-3a	Sigma	Cat#SML0580-5MG
Doxycycline	Sigma	Cat#D3447-500MG
Blasticidin	Thermo	Cat#A1113903
Puromycin	Thermo	Cat#A1113803
Critical Commercial Assays
QIAGEN RNeasy Mini Kit isolation	QIAGEN	Cat#74106
NEBNext® Poly(A) mRNA Magnetic Isolation Module	NEB	Cat#E7490
NEBNext® UltraII Directional RNA Library Prep Kit	NEB	Cat#E7760
NEBNext Ultra II DNA Library Prep Kit	NEB	Cat#E7645
NEBNext Lib Quant Kit	NEB	Cat#E7630
High-Capacity RNA to cDNA kit	Thermo	Cat#4368813
Deposited Data
SON TSA-seq raw and analyzed data	this paper	GSE154095
RNA-seq raw and analyzed data	this paper	GSE139003
Experimental Models: Cell Lines
Human: Passage 25–35 IMR90 primary lung fibroblast cell line	ATCC	Cat#CL186
Human: Saos2 cells	ATCC	Cat#HTB-85
Human: 293T cells	ATCC	Cat#CRL-3216
Oligonucleotides
Primers for ChIP-qPCR, see Table S2	this paper	N/A
Primers for qRT-PCR, see Table S2	this paper	N/A
Recombinant DNA
Lenti CMV rtTA3 Blast (w756-1)	Eric Campeau Addgene	Cat#26429; RRID: Addgene_26429
pVXL-TRE3G	Takara	Cat#631187
pVXL-TRE3G p53 wild type	this paper	N/A
pVXL-TRE3G p53 L22Q, W23S	this paper	N/A
pVXL-TRE3G p53 W53Q, F54S	this paper	N/A
pVXL-TRE3G p53 L22Q, W23S, W53Q, F54S	this paper	N/A
pVXL-TRE3G p53 Δ62–77	this paper	N/A
pVXL-TRE3G p53 Δ62–91	this paper	N/A
pVXL-TRE3G p53 R273H	this paper	N/A
pVXL-TRE3G p53 L344P	this paper	N/A
pVXL-TRE3G p53 370*	this paper	N/A
psPAX2	Didier Trono Addgene	Cat#12260; RRID: Addgene_12260
pVSV-G	Didier Trono Addgene	Cat#12259; RRID: Addgene_12259
pLKO.1 SON shRNA	Sigma	TRC ID: TRCN0000083723
pLKO.1 SRSF1 shRNA1	Sigma	TRC ID: TRCN0000010592
pLKO.1 SRSF1 shRNA2	Sigma	TRC ID: TRCN0000001093
Software and Algorithms
Rajlabimagetools for analysis of spots from RNA- and DNA-FISH data	Mellis et al., 2017	https://github.com/arjunrajlaboratory/rajlabimagetools/wiki
Blob analysis module of rajlabimagetools for analysis of nuclear speckles	this paper	https://github.com/arjunrajlaboratory/rajlabimagetools/wiki/Blob-Analyzer
Use of rajlabimage tools for semi-manual measurement of loci distance to speckle from RNA-FISH and immunoDNA-FISH data	this paper	https://github.com/katealexander/distanceToSpeckle-semiManual
STAR	Dobin et al., 2013	https://github.com/alexdobin/STAR/releases
Picard	“Picard Toolkit.” 2019. Broad Institute, GitHub Repository.	https://broadinstitute.github.io/picard/
htseq	Anders et al., 2015	https://htseq.readthedocs.io/en/master/
DESeq2	Love et al., 2014	http://www.bioconductor.org/packages/release/bioc/html/DESeq2.html
Bowtie2	Langmead and Salzberg, 2012	http://bowtie-bio.sourceforge.net/bowtie2/index.shtml
DiffBind	Wu et al., 2015	https://bioconductor.org/packages/release/bioc/html/DiffBind.html
Use of DiffBind for SON TSA-seq of sliding windows	this paper	https://github.com/katealexander/TSAseq-Alexander2020/tree/master/genomicBins_DiffBind
BEDTools	Quinlan and Hall, 2010	https://bedtools.readthedocs.io/en/latest/
clusterProfiler	Yu et al., 2012	https://bioconductor.org/packages/release/bioc/html/clusterProfiler.html
Fiji	Schindelin et al., 2012	https://imagej.net/Fiji