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. 2022 Jan 27;9:802794. doi: 10.3389/fbioe.2021.802794

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Copyright © 2022 Lan, Huang, Fu, Liu, Zhang, Li and Yang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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OCNN for drug screening. (A) The predicted osteogenic score (POS) of the rBMSCs cultured with different concentrations of simvastatin on day 1 and Alizarin red staining on day 14 was quantified by ImageJ. Linear fits were performed using the POS and Alizarin red staining areas to calculate the correlation, p < 0.05. (B) The predicted osteogenic score (POS) of the rBMSCs cultured with different concentrations of alendronate sodium on day 1 and Alizarin red staining on day 14 was quantified by ImageJ. Linear fits were performed using the POS and Alizarin red staining areas to calculate the correlation, p<0.05. (C) The predicted osteogenic score (POS) of the rBMSCs cultured with different concentrations of VD3 on day 1 and Alizarin red staining on day 14 was quantified by ImageJ. Linear fits were performed using the POS and Alizarin red staining areas to calculate the correlation, p<0.05. (D) The predicted osteogenic score (POS) of the rBMSCs cultured with different concentrations of BMP-2 on day 1 and Alizarin red staining on day 14 was quantified by ImageJ. Linear fits were performed using the POS and Alizarin red staining areas to calculate the correlation, p<0.05.