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. 2021 Oct 5;28(4):457–470. doi: 10.3390/pathophysiology28040029

Figure 2.

Figure 2

NR4A1 muscle myotubes—ACEA, rimonabant, ACEA and rimonabant, and insulin affect NR4A1 gene expression; myotubes fed with delipidated serum. The stimulation time covered 3 to 24 h, and NR4A1 mRNA levels, relative to TATA-Box, was evaluated by quantitative real-time PCR (qRT-PCR) (100 nM). The following scenarios explain the stimulation process conducted: (a) Stimulation was done for 1 h. (b) Stimulation was done for 3 h. (c) Stimulation was applied for up to 5 h. (d) Stimulation was applied for up to 24 h. The data were reported as the mean ± SEM of three separate groups. (n = 3; * denotes p < 0.05, ** denotes p < 0.01, and *** denotes p < 0.001). Data were investigated by conducting one-way ANOVA test and Tukey test. NR4A1; nuclear receptor subfamily 4, group A, member 1.