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. 2021 Oct 5;28(4):457–470. doi: 10.3390/pathophysiology28040029

Figure 4.

Figure 4

NR4A3 muscle myotubes—ACEA, rimonabant, ACEA and rimonabant, and insulin affect NR4A3 gene expression; myotubes fed with delipidated serum. The stimulation time covered 3 to 24 h, and NR4A3 mRNA levels, relative to TATA-Box, was evaluated by quantitative real-time PCR (qRT-PCR) (100 nM). The following scenarios explain the stimulation process conducted: (a) Stimulation was done for 1 h. (b) Stimulation was done for 3 h. (c) Stimulation was applied for up to 5 h. (d) Stimulation was applied for up to 24 h. The data were reported as the mean ± SEM of three separate groups. (n = 3; * denotes p < 0.05, and ** denotes p < 0.01). Data were investigated by conducting one-way ANOVA test and Tukey test. NR4A3; nuclear receptor subfamily 4, group A, member 1.