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. 2022 Jan 27;12:793449. doi: 10.3389/fphar.2021.793449

FIGURE 5.

FIGURE 5

Palmatine inhibited colon cancer in vitro and in vivo. (A,B) CCK8 assay of WT (blue) and MSI2−/− (red) HCT116 (A) or A549 (B) cells upon treatment of palmatine for 72 h at indicated doses. Quantifications of three independent experiments were represented as means ± SD. (C) Colony formation of HCT116, RKO, and SW480 cells. Cells were treated with palmatine or Ro 08-2750 with indicated concentrations for 8 days before staining with crystal violet; colony formation was quantified by OD 590 nm measurement and was normalized to the WT. (D) HCT116 cells xenograft tumors’ weight treated with palmatine as indicated for 13 days. p < 0.01, unpaired t-test (two tailed). (E) Mice weight of HCT116 cells xenograft nude mice treated with palmatine as indicated for 13 days. (F) Growth curves of HCT116 cells xenograft tumors generated by palmatine treatment in nude mice. p < 0.05, two-way ANOVA. Data were presented as mean ± SD of six mice in each group.