Skip to main content
. 2021 Jun 16;43(6):488–500. doi: 10.1093/eurheartj/ehab222

Figure 2.

Figure 2

Smooth muscle-specific TBCE deficiency leads to endothelial dysfunction, vascular stiffness and increased vessel wall thickness. (A–G) Concentration/relaxation curves of isolated aortic rings. (A) Maximal contraction (g) in response to PGF2α. (B and C) Vascular tension (g) curves in response to ACh and GTN (Two-way ANOVA, Tukey’s multiple comparison test). Vascular concentration-relaxation curves in response to ACh and GTN of sham-operated (D and F) and AngII infused (E and G; 1 mg/kg/day) mice (n = 6–8 per group) (two-way Student’s t-test). (H) Representative ultrasound images of B-mode, M-mode and pulsed-wave Doppler of the murine aortas and graphs of left common carotid artery (LCCA). (I) Resistance index and (J) Pulsatility index (n = 5–6 per group). (K) Sirius red (collagen) and haematoxylin–eosin staining of murine aortas and graphs of L. Media thickness (µm) and (M) Collagen/media thickness (n = 4–5 per group); bars represent 20μm and images were gained at a 40 × magnification (n = 3–5 per group) (one-way ANOVA, Tukey’s multiple comparison test).