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. 2021 Jun 16;43(6):488–500. doi: 10.1093/eurheartj/ehab222

Figure 5.

Figure 5

The chemical chaperone TUDCA reverses vascular dysfunction and VSMCs proliferation in mice with SMC-specific TBCE deficiency. Concentration/relaxation curves of isolated aortic rings, in response to (A) ACh and (B) GTN (Two-Way ANOVA, Tukey’s multiple comparison test) (C) Maximal contraction (g) in response to PGF2α (D) Representative western blot images and (E) Graph of relative densitometry analysis of ER stress and autophagy markers of the murine aortas. (F) MTT absorbance (570 nm) graph of the SMMHC-ERT2-Cre+/−TBCEwt/wt and SMMHC-ERT2-Cre+/−TBCEfl/fl VSMCs with/without TUDCA treatment (1 mM) (G) Confluency images of the primary VSMCs in culture with/without TUDCA treatment (1 mM). (H) Representative Western blot images and I. graph of relative densitometry analysis of ER stress and autophagy markers of the primary VSMCs normalized to α-actinin. (Tukey’s multiple comparison test; 1 way ANOVA; Different n numbers in the in vitro experiments refer to individual experiments; Dotted line on western blot images represent a readjustment of the representative image).