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. Author manuscript; available in PMC: 2023 Feb 15.
Published in final edited form as: J Immunol. 2022 Jan 19;208(4):861–869. doi: 10.4049/jimmunol.2100481

Figure 6. Absence of IL-36R resulted in reduced regulatory T cells in the liver following Con A injection.

Figure 6.

WT and IL-36R−/− mice were i.v. treated with 12 mg/kg of Con A. PBS-injected mice were used as controls (0 h). Intrahepatic lymphocytes and RNA were obtained from the livers at 12 and 24 h after Con A treatment. (A) Percentages and numbers of Foxp3+ CD4+ Treg cells were analyzed in the livers by flow cytometry. (B) Total RNAs were extracted from the liver at 12 and 24 h after Con A treatment. Expressions of CCL5, CCL17 and CXCL12 mRNA in the liver were determined by qRT-PCR. n= 4–5 samples/ control group. n = 9–12 samples/ Con A-group from pooled experiments. The data are shown as mean ± SEM of each group from three independent experiments. Two-tailed unpaired T test was used for statistical analysis. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001.