Circumferences and extracellular water in lower extremity. Percent changes in circumferences of the calf (A) and the malleolus (B) in the control (without garment, open symbols) and garment (closed symbols) legs before and during 8 h of constant sitting. C, Percent changes in extracellular water content of lower extremity muscles in the control (open bar) and garment (closed bar) legs after 8-h constant sitting. D, Correlation between percent changes in extracellular water content of lower extremity muscles and percent changes in calf circumferences in the control (open symbols) and garment (closed symbols) legs after 8-h constant sitting. A–C, Group data expressed as the mean ± SD. D, Individual data of nine healthy males. A, B, Significance for an effect of sitting time (baseline vs 1, 2, 3, 4, 5, 6, 7, and 8 h of sitting) or elastic garment (with or without) was quantified with a two-way ANOVA for repeated measures, followed by Bonferroni post hoc testing as appropriate. C, Wilcoxon’s signed-rank test was performed between the control and garment legs. D, Spearman correlations between changes in extracellular water content of lower extremity muscles and changes in calf circumferences after 8-h constant sitting were determined. *P < 0.05 between baseline and each time point of sitting within a group; †P < 0.05 between the control and the garment leg at the same time point.