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. Author manuscript; available in PMC: 2022 Feb 10.
Published in final edited form as: Nat Protoc. 2020 Dec 4;16(1):327–351. doi: 10.1038/s41596-020-00418-9

Table 2.

Custom siRNA sequences

siRNA Sense (5′–3′) Antisense (5′–3′)
HIV-1 tat/rev site I 27 mer DsiRNA (viral RNA) GCG GAG ACA GCG ACG AAG AGC UCA UCA UGA UGA GCU CUU CGU CGC UGU CUC CGC dTdT
Human CD4 21-mer siRNA GAU CAA GAG ACU CCU CAG U dGdA ACU GAG GAG UCU CUU GAU C dTdG
Human CD4 27-mer DsiRNA. GAU CAA GAG ACU CCU CAG UGA GAA G CUU CUC ACU GAG GAG UCU CUU GAU CUG-3’ (2′-OMe modified U is underlined.)
Mouse JAK1 21-mer siRNA GAA UAA AUG CAG UAU CUA AAU P-UUA GAU ACU GCA UUU AUU CGG (a phosphate is added in 5′ and underlined.)