Table 1.
Sequences and concentrations of each primer and probe used in RT-qPCR
Target | Name | Sequence (5′–3′) | Final conc. (μM) | References |
---|---|---|---|---|
OC43 | OC43-FP | ATGTTAGGCCGATAATTGAGGACTAT | 0.3 | Vijgen et al. (2005) |
OC43-RP | AATGTAAAGATGGCCGCGTATT | 0.3 | ||
OC43-TP | FAM-CATACTCTGACGGTCACAAT-NFQ-MGB | 0.2 | ||
OC43-FPT7 | TAATACGACTCACTATAGGGAGGATGTTAGGCCGATAATTGAGGACTAT | 0.3 | ||
SARS-CoV-2 N1 | N1-F | GACCCCAAAATCAGCGAAAT | 0.2 | US Centers for Disease Control and Prevention (2020) |
N1-R | TCTGGTTACTGCCAGTTGAATCTG | 0.2 | ||
N1-P | FAM-ACCCCGCAT-ZEN-TACGTTTGGTGGACC-3IABkFQ | 0.2 | ||
SARS-CoV-2 N2 | N2-F | TTACAAACATTGGCCGCAAA | 0.2 | US Centers for Disease Control and Prevention (2020) |
N2-R | GCGCGACATTCCGAAGAA | 0.2 | ||
N2-P | HEX-ACAATTTGC-ZEN-CCCCAGCGCTTCAG-3IABkFQ | 0.2 |