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. 2022 Feb 3;2022:7471246. doi: 10.1155/2022/7471246

Figure 2.

Figure 2

Characterization of ADSCs and ADSC-Exos. (a) Adipogenic differentiation of ADSCs was confirmed by Oil Red O staining. Scale bar = 100 μm. (b) Osteogenic differentiation of ADSCs was confirmed by Alizarin Red S staining. Scale bar = 50 μm. (c) Chondrogenic differentiation of ADSCs was confirmed by Alcian Blue staining. The red arrow indicates the micromass of ADSCs. Scale bar = 100 μm. (d) Nanoparticle size distribution of ADSC-Exos was analyzed by NTA. (e) The morphology of ADSC-Exos was observed by TEM. Scale bar = 100 nm. (f) Western blotting was used to identify exosome-specific markers (TSG101, CD9, and CD81). CM-NExo: exosome-depleted culture medium; Exo: ADSC-Exos.