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. 2022 Jan 28;9:828577. doi: 10.3389/fbioe.2021.828577

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Copyright © 2022 Moerman, Korteland, Dilba, van Gaalen, Poot, van Der Lugt, Verhagen, Wentzel, van Der Steen, Gijsen and Van der Heiden.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A–E). Selection of typical histology cross-sections of carotid plaques seen when moving from proximal to distal along the length of the carotid plaque. Cross-sections (A–C) are seen proximal to the flow divider and (D,E) distal to the flow divider. Row 1: Miller’s elastic stain. Row 2: segmentation of the necrotic core (NC), fibrin, and artifact, based on the combination of histochemical staining procedures performed. Row 3: CD68⁺ immunohistochemical stain. CD68-positive areas are marked in red. Row 4: segmentation of CD68⁺ stained slides, showing macrophage areas, NC, and artifacts.