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Homogeneous glycoforms of normal polyclonal IgG prepared by chemoenzymatic glycoengineering. (A) SDS-PAGE of the glycoforms of IgG. All the IgG glycoforms including the native protein used in this study were purified by protein G affinity chromatography. (B) HILIC-UPLC analysis of glycans released from the Fc fragments of IgG glycoforms. The peaks of the oligosaccharides of native IgG are listed in Supplementary Table 1 .
