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. 2022 Feb 10;13:791. doi: 10.1038/s41467-022-28452-z

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Stable silencing of ENSA expression in the TNBC cell lines BT549 and MDA-MB-231. b In vitro growth curves of BT549 and MDA-MB-231 cells expressing control or ENSA shRNA. n = 6. Data are presented as mean ± SD. Two-tailed two-way ANOVA tests. c Colony formation of BT549 and MDA-MB-231 cells expressing control or ENSA shRNA. n = 3. Data are presented as mean ± SD. Two-tailed unpaired Student’s t tests. d Colony formation of BT549 and MDA-MB-231 cells ± ENSA knockdown and ± ENSA overexpression. n = 3. Data are presented as mean ± SD. Two-tailed unpaired Student’s t tests. e In vitro growth curves of BT549 and MDA-MB-231 cells ± ENSA knockdown and ± ENSA overexpression. n = 6. Data are presented as mean ± SD. Two-tailed two-way ANOVA tests. f Apoptosis levels were measured in BT549 and MDA-MB-231 cells expressing control or ENSA shRNA. Percentage of annexin V+ cells are shown. n = 3. Data are presented as mean ± SD. Two-tailed unpaired Student’s t tests. Source data are provided as a Source Data file.