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. 2022 Feb 10;13:800. doi: 10.1038/s41467-022-28343-3

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a IF of KRT82 (green) in postnatal (p) mouse skin. Mouse HFs are in telogen at postnatal days 22 and 90 (p22, p90), in anagen at p31, and catagen at p43. Cuticular staining of KRT82 is only observed in the p31 (anagen) HF. White dashed line outlines the hair shaft cuticle. Weak staining observed in mouse epidermis may be a result of green autofluorescent properties of skin epidermal cells. IF staining was repeated at 3 other anagen timepoints and 2 additional telogen timepoints with similar results. b Western blot analysis of whole mouse skin at varying stages of the hair cycle shows that keratin protein is only present in the anagen phase (p31) of the mouse hair cycle. Western blot was repeated with similar results 2 additional times. Source data are provided as a Source data file.