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. 2022 Feb 10;12:2301. doi: 10.1038/s41598-022-06274-9

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Ion chromatograms of representative samples from the normal control and the individual cancer groups. The total run time for the LC resolution was 14 min, with every sample run being alternated with a blank run. For the blank run involved injection of a 1:1 mixture of methanol and water. Comparatively, spectra in each case were following a trend with major changes seen from 200 to 600 m/z with the time ranging from 3 to 11 min.