Fig. 3. Properties of ONE-modified and unmodified oligomers of truncated αSN mutants.

a–d SAXS analysis of C-terminal truncated αSN oligomers. a, b The data with a previously developed model for αSO140 suggest similar ellipsoidal shapes for all oligomers, i.e., a dense core and diffuse shell. However, parameters related to the shape of core and shell change in different ways in truncated constructs of unmodified versus ONE-modified αSOs. c The number of amino acids in the shell fluctuate in unmodified αSOs but decrease with truncation in ONE-modified αSOs. d There is also an increase in the number of monomers per oligomer for both kinds of oligomers. Each point is the average of three replicates where error bars show standard deviations. e Schematic model of unmodified αSN FL oligomers based on SAXS³². A compact core is surrounded by a shell of more flexible polypeptide chains.