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. 2022 Jan 28;9:784626. doi: 10.3389/fbioe.2021.784626

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Copyright © 2022 Woo, Lee, Lee, Ku, Park and Cho.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Schematization of the Punch-based Assistance Clarity-measurement Assay (PACA) platform. 1) Sample preparation for measuring tissue transparency. Cleared or uncleared samples were punched using metal eyelets, either 3.5 or 6 mm in diameter. 2) PACA-Light: Each disc was transferred to an individual well of a clear 96-well plate. An ELISA reader spectrophotometer was used to measure light absorbance along a range of wavelengths. Transmittance was analyzed based on the obtained absorbance values. 3) PACA-Glow: Each disc was transferred to an individual well of a black, opaque 96-well plate with a luminous disk (SrAl₂O₄:Eu²⁺, Dy³⁺ photoluminescent). A gel documentation system was used to analyze the transmission of light from the luminous disk through the disc. Details of the process applied are provided in Supplementary Figure S1.