TABLE 1.

Comparison of tissue clearance achieved in rat brains processed via various tissue clearing methods in this study.

Tissue clearing method	3-mm thick rat brain slice
	PACA-light						PACA-glow
	Minimal transmittance (%)						Transmittance (%)
	OD 600			OD 850			30-100 s
	B1	B2	B3	B1	B2	B3	B1	B2	B3
psPACT	±48	±37	±25	±86	±77	±57	±66	±50	±43
mPACT	±47	±40	±34	±83	±83	±72	±89	±85	±76
SWITCH-1	±51	±50	±46	±78	±76	±67	±76	±75	±70
SWITCH-4	±90	±81	±78	±95	±90	±87	±92	±89	±86
ClearT	±4	±3	±2	±10	±8	±4	±12	±9	±4
ClearT2	±2.5	±1	±0.5	±9	±2	±1	±25	±9	±6
ScaleA2	±7	±6.5	±1	±20	±18	±2	±52	±41	±19
ScaleS	±8	±8	±5	±27	±25	±20	±23	±18	±16
CUBIC-L/R	±75	±74	±65	±96	±93	±89	±88	±83	±68
CUBIC-X	±64	±64	±60	±83	±82	±80	±74	±71	±63
FOCM	±9	±10	±6	±30	±24	±17	±25	±20	±8
RTF	±1.5	±1	±1	±6	±4	±3	±10	±9	±8
Ce3D	±5	±3	±1	±13	±10	±2	±8	±7	±2
MACS	±31	±30	±4	±53	±53	±11	±52	±45	±22
SeeDBp	±14	±13	±4	±38	±36	±11	±27	±22	±17
SeeDB	±2.5	±2	±1	±13	±10	±5	±12	±10	±3
SeeDB2	±1	±1	±0.5	20	±11	±7	±12	±5	±5
80% TDE	±14	±8	±2	±49	±33	±6	±30	±18	±4
LUCID	±13	±17	±3	±44	±47	±12	±38	±37	±16
OPTIClear	±15	±16	±4	±31	±28	±7	±32	±28	±16
BABB	±87	±83	±76	±97	±94	±92	±82	±74	±61
1P-BABB	±71	±51	±40	±91	±84	±63	±68	±55	±40
tB-BABB	±59	±63	±48	±77	±88	±81	±66	±53	±37
3DISCO	±71	±63	±29	±94	±91	±51	±67	±57	±21
iDISCO+	±75	±71	±39	±95	±91	±60	±90	±81	±55
uDISCO	±47	±28	±20	±73	±42	±48	±85	±63	±60
FDISCO	±63	±63	±55	±94	±94	±88	±84	±81	±61
vDISCO	±72	±67	±70	±90	±90	±87	±82	±82	±79
Ethanol-ECi	±54	±30	±34	±99	±87	±51	±77	±72	±40
PEGASOS	±62	±51	±43	±92	±81	±85	±78	±68	±51