Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Jan 28;12:796060. doi: 10.3389/fgene.2021.796060

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2022 Kute, Soukarieh, Tjeldnes, Trégouët and Valen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Profiling and sequencing of translating transcripts. A254 profiles shown before (A) and after digestion with ribonucleases (B,C). The fractions used for further processing are highlighted, polysomes in purple, 80S in orange and 40S in green. (D) The process of library preparation for next generation sequencing. Size selection of ∼30 nt is done for ribosome profiling and ribosome complex profiling sequencing and libraries are prepared from the size selected small RNAs, whereas for polysome profiling, libraries are prepared from total RNA. Meta-coverage shown for reads obtained from polysome profiling sequencing (E), for ribosome profiling (F) and for ribosome complex profiling [(G) top: 40S, bottom: 80S].