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. 2022 Jan 28;12:781282. doi: 10.3389/fphar.2021.781282

FIGURE 1.

FIGURE 1

LPS promotes the migration and proliferation of HDF and MT depolymerization. (A) Scratch wound healing assays were performed to examine HDF migration with or without LPS (5, 10, 50, 100, 500 and 1,000 ng/ml) for 24 h. Representative pictures of the scratch wound are shown. Bar, 200 μm, and (B) the images were quantitatively analyzed (n = 5). (C) HDF was subjected to LPS (500 ng/ml) for 12, 24 and 36 h to detect cell proliferation using the CCK-8 assay (n = 6). (D) HDF proliferation was detected after 24 h with or without LPS by Edu staining and (E) quantitative analysis (n = 5). Nuclei were stained with Hoechst 33342. The merged image is to show the proportion of proliferating cells (green) to total cells (blue). Bar, 20 µm. (F) Western blotting was performed to detect PCNA with or without LPS (500 ng/ml) treatment and (G) quantitative analysis (n = 5). (H) Representative confocal tubulin immunofluorescence images showing the MT structure with or without LPS (500 ng/ml) for 24 h. The inserts show high-magnification images of the MT network. Bar, 10 µm. LPS, lipopolysaccharide; PCNA, proliferating cell nuclear antigen. The results are shown as means ± SEM. * p < 0.05 was considered to be significant.