FIGURE 2.

Involvement of MT depolymerization in LPS-induced HDF migration and proliferation. (A) Scratch wound healing assays were performed to detect HDF migration treated with LPS with or without taxol (1 µm) pretreatment, Bar, 200 μm, and (B) quantitative analysis (n = 5). (C) HDF proliferation was detected after LPS treatment with or without taxol pretreatment by Edu staining and (D) quantitative analysis (n = 5). Nuclei were stained with Hoechst 33342. The merged image is to show the proportion of proliferating cells (green) to total cells (blue). Bar, 20 µm. (E) For the tubulin immunofluorescence images of MTs, cells were pretreated with taxol before LPS stimulation. These inserts show high-magnification images of the peripheral MT network. Bar, 10 µm. (F) HDF was subjected to LPS with or without taxol pretreatment to detect cell proliferation using the CCK-8 assay (n = 10). (G) Western blotting was performed to detect PCNA after LPS treatment with or without taxol, and (H) the results were quantitatively analyzed (n = 5). The data are represented as the mean ± SEM. * p < 0.05 was considered to be significant.