FIGURE 3.

LPS-induced stathmin expression and its effects on HDF migration and proliferation and MT depolymerization. (A) Western blotting was performed to detect stathmin expression with or without LPS, and (B) the results were quantitatively analyzed (n = 5). (C) Western blotting was performed to detect stathmin expression in wound edge tissue post wounding, and (D) the results were quantitatively analyzed (n = 5). (E) Stathmin expression at the wound edge of mice post wounding was detected by immunohistochemical staining. Bar, 100 μm, the arrow points to the positive cells, and (F) the results were quantitatively analyzed (n = 5). (G) Cells were transfected with siSTMN or siNC before treatment with or without LPS stimulation. Western blotting was performed to detect stathmin and PCNA expression, and (H) the results were quantitatively analyzed (n = 5). (I) Scratch wound healing assays were performed to detect HDF migration treated with LPS after siSTMN or siNC transfection., Bar, 200 μm, and (J) the results were quantitatively analyzed (n = 5). (K) For the tubulin immunofluorescence images of MTs, cells were transfected with siNC or siSTMN before LPS treatment. The inserts show high-magnification images of the peripheral MT network. Bar, 10 µm. (L) The cells were transfected with siSTMN or siNC before LPS stimulation and detected by Edu staining, and (M) the results were quantitatively analyzed (n = 5). Nuclei were stained with Hoechst 33342. The merged image is to show the proportion of proliferating cells (green) to total cells (blue). Bar, 20 µm. (N) HDF proliferation was tested using the CCK-8 assay (n = 10). siSTMN, small interfering RNA for stathmin; siNC, small interfering RNA for negative control. The results are shown as means ± SEM. * p < 0.05 was considered to be significant.