FIGURE 5.

Role of p38/MAPK activation in LPS-induced HDF migration and proliferation and MT depolymerization. (A) Scratch wound healing assays were performed to detect HDF migration treated with LPS with or without SB (5 µm) pretreatment. Bar, 200 μm, and (B) the results were quantitatively analyzed (n = 5). (C) HDF proliferation was detected after LPS treatment with or without SB pretreatment by Edu staining, and (D) the results were quantitatively analyzed (n = 5). Nuclei were stained with Hoechst 33342. The merged image is to show the proportion of proliferating cells (green) to total cells (blue). Bar, 20 µm. (E) For the tubulin immunofluorescence images of MTs, cells were pretreated with SB before LPS treatment. These inserts show high-magnification images of the peripheral MT network. Bar, 10 µm. (F) HDF was subjected to LPS treatment with or without SB pretreatment to detect cell proliferation using the CCK-8 assay (n = 10). (G) Western blotting was performed to detect PCNA expression after LPS treatment with or without SB, and (H) the results were quantitatively analyzed (n = 5). The data are represented as the mean ± SEM. * p < 0.05 was considered to be significant.