FIGURE 6.

P38/MAPK induced HDF migration and proliferation via stathmin-dependent MT depolymerization. (A) Western blotting was performed to analyze the effects of MKK6(Glu) transfection for the indicated times, and (B) the results were quantitatively analyzed (n = 3). (C) Western blotting was performed to detect PCNA expression after the cells were transfected or cotransfected with siSTMN or MKK6(Glu), and (D) the results were quantitatively analyzed (n = 5). (E) Scratch wound healing assays were performed to detect HDF migration after cells were transfected or cotransfected with siSTMN or MKK6(Glu). Bar, 200 μm, and (F) the results were quantitatively analyzed (n = 5). (G) HDF proliferation was detected after the cells were transfected or cotransfected with siSTMN or MKK6(Glu) by EdU staining, and (H) the results were quantitatively analyzed (n = 5). Nuclei were stained with Hoechst 33342. The merged image is to show the proportion of proliferating cells (green) to total cells (blue). Bar, 20 µm. (I) For the tubulin immunofluorescence images of MTs, cells were transfected or cotransfected with siSTMN or MKK6(Glu). These inserts show high-magnification images of the peripheral MT network. Bar, 10 µm. (J) Cell proliferation was detected after the HDF was transfected or cotransfected with siSTMN or MKK6(Glu) using the CCK-8 assay (n = 10). The results are shown as the means ± SEM. * p < 0.05 was considered to be significant.