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. 2022 Jan 28;13:806224. doi: 10.3389/fneur.2022.806224

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Copyright © 2022 Fang, Tong and Lu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Demonstration of GFAP-IgG by GFAP-transfected HEK293 cell-based immunofluorescence assay. (A,C,E) HEK293 cells expressing green fluorescent protein (GFP)-tagged GFAP (green). (B,D,F) HEK293 cells immunostained (red if positive); (B) Positive control with human anti-GFAPα IgG. (D) Negative with healthy control. (F) Positive result of cerebrospinal fluid (CSF) (titer at 1:32). Scale bar = 50 μm.