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. 2022 Jan 28;9:789621. doi: 10.3389/fbioe.2021.789621

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Copyright © 2022 Pattappa, Reischl, Jahns, Schewior, Lang, Zellner, Johnstone, Docheva and Angele.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Representative photomicrographs of hyperoxia (HYP) and physioxia (PHY) expanded NFA (A) avascular and (B) vascular meniscus cells with corresponding population growth curves (n = 7; mean ± S.D.). (C) Representative photomicrographs of Oil-red-O staining for lipid droplet and alizarin red staining for calcium deposition for meniscus populations under hyperoxia (HYP) and physioxia (PHY). Pellet (D) wet weight (E) GAG content for avascular and vascular meniscus cells with representative (D) macroscopic and (E) DMMB-stained pellets.