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. 2022 Jan 28;9:789621. doi: 10.3389/fbioe.2021.789621

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Copyright © 2022 Pattappa, Reischl, Jahns, Schewior, Lang, Zellner, Johnstone, Docheva and Angele.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) Representative crystal violet-stained colonies cultured under hyperoxia (HYP) and physioxia (HYP) on uncoated (PL) and fibronectin (FN)-coated dishes. (B) Number of colonies counted from conditions described in (A) from avascular and vascular meniscus cells (n = 6; data represent mean ± S.D.; *p < 0.05). (C) Representative photomicrographs of a fibronectin colony and avMPCs and vMPCs cultured under hyperoxia and physioxia with (D) population growth curves for expanded avMPC and vMPC population under hyperoxia and physioxia [n = 4 (3 clones/donor); data represent mean ± S.D.]. (E) Representative Oil-red-O staining for lipid droplet formation and alizarin red staining for calcium deposition for avMPCs and vMPCs.